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1.Helena Persson, Hilde Nybom - Genetic Sex Determination and RAPD Marker Segregation in the Dioecious Species Sea Buckthorn (Hippophae Rhamnoides L.)
2.Helena Persson, Hilde Nybom - RAPD Analysis of Genetic Variation Within and Among Populations of Turk's Cap Lily (Lilium Martagon L.)
3.Igor V Bartish, H Nybom, Niklas Jeppsson - Population genetic structure in the dioecious pioneer plant species Hippophae rhamnoides investigated by random amplified polymorphic DNA (RAPD) markers Molecular Ecology,1999
4.Igor V Bartish, Hilde Nybom - Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants Perspectives in Plant Ecology Evolution and Systematics, 2000
5. Maria Lovato, Valéria Freitas - Contrasting genetic diversity and differentiation of populations of two successional stages in a Neotropical pioneer tree (Eremanthus erythropappus, Asteraceae) Genetics and Molecular Research, 2008;
6. Igor V Bartish Helena Persson Niklas Jeppsson - DNA Analysis as a Tool in Sea Buckthorn Breeding 1999;
7.Saji Gomez Deepu Mathew T. Parimelazhagan Characterization of Seabuckthorn (Hippophae spp.) genetic resources in India using morphological descriptors 2007;
8. Amit Sharma, Gaurav Zinta, Satender Rana, Poonam Shirkot Molecular identification of sex in Hippophae rhamnoides L.using isozyme and RAPD markers;
9. Marius Ekué - Population Genetics of the Cycad Encephalartos Barteri Ssp. Barteri (Zamiaceae) In Benin With Notes on Leaflet Morphology and Implications for Conservation, 2008;
10. Satender Rana A Female Sex Associated Randomly Amplified Polymorphic DNA Marker in Dioecious Hippophae salicifolia Global Science Books;
11. Anita Grewal Marker assisted sex differentiation in dioecious plants JPR  Solutions;
12. MANU PANT*, ANKITA LAL, ANJU RANI - HIPPOPHAE SALICIFOLIA D DON- A PLANT WITH MULTIFARIOUS BENEFITS International Journal of Pharmacy and Pharmaceutical Sciences;
13. Amit Sharma Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers Forestry Studies in China, 2010;
14. Virendra Singh Fatty acids, tocols, and carotenoids in pulp oil of three sea buckthorn species (Hippophae rhamnoides, H. salicifolia, and H. tibetana) grown in the Indian Himalayas Journal of the American 2006;
15. Manju Sundriyal, Lalit Kumar, V. Purohit, R. Maikhuri, K. Saxena, S.V.C Kameswara Ra Basic nutritional attributes of Hippophae rhamnoides (Seabuckthorn) populations from Uttarakhand Himalaya, India 2000;
16. Y.C. Tripathi, Nishat  Anjum Flavonoid Constituents, Total Polyphenol and Antioxidant Efficacy of Hippophae rhamnoides L. Berries;
17. Raghava ReddyPHYTOCHEMICAL INVESTIGATIONS ON SEA BUCKTHORN (Hippophae rhamnoides) BERRIES;
18. Jan Oszmiański Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn ( Hippophaë rhamnoides L.) Berries Journal of Agricultural and Food Chemistry, 2015;
19. Somen Acharya Seabuckthorn (Hippophae sp. L.): New crop opportunity for biodiversity conservation in cold arid Trans-Himalayas, 2010;         
20. Sushen Pradhan Seabuckthorn (Hippophae sp. L.): New crop opportunity for biodiversity conservation in cold arid Trans-Himalayas;
21. Tsering Stobdan Germination and short-term storage of Hippophae rhamnoides L. seeds and its ex-situ reintroduction potential assessment under North East Indian conditions;
22. Saurabh Chaudhary Prakash Sharma Ankit Jain Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome, Stress physiology and molecular biology of plants;
23. Rajesh Ghanga, Saurabh Raghuvanshi, Prakash C. Sharma Expressed sequence tag based identification and expression analysis of some cold inducible elements in seabuckthorn (Hippophae rhamnoides L.) Plant Physiology and Biochemistry 2012;
24. Rajesh Ghangal Saurabh Chaudhary Ram Purty Mukesh Jain Opti Prakash Sharma Optimization of De Novo Short Read Assembly of Seabuckthorn (Hippophae rhamnoides L.) Transcriptome; LoS ONE, 2013;
25. Yannick Weesepoel, Carmen Socaciu, Adela Pintea, Raluca Pop, Harry Gruppen Carotenoid composition of berries and leaves from six Romanian sea buckthorn (Hippophae rhamnoides L.) varieties Food Chemistry
25. S. Cenkowski R. Yakimishen, R. Przybylski W.E. Muir Quality of extracted sea buckthorn seedand pulp oil;
Department of Biosystems Engineering, University of Manitoba, Winnipeg, Manitoba R3T 5V6, Canada; and Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge,AlbertaT1K3M4,Canada. CANADIAN BIOSYSTEMS ENGINEERING3.9Volume48 2006*
Email:stefan_cenkowski@umanitoba.ca
26. Kottapalli s. Rao, R. Maikhuri, Shalini Dhyani, Srikant Misra, D. Dhyani Endorsing the declining indigenous ethnobotanical knowledge system of Seabuckthorn in Central Himalaya, India Journal of Ethnopharmacology, 2010;
27. Ashok Singh, Jitendra S. Butola,S. S. Samant, Pankaj Sharma, ManoharLal & Sunil Marpa Indigenous Techniques of Product Development and Economic Potential of Seabuckthorn: A Case Study of Cold Desert Region of Himachal Pradesh, India Proceedings of the NationalAcademy of Sciences, India Section B:Biological Sciences
 ISSN 0369-8211Volume 82Number 3 Proc. Natl. Acad. Sci., India, Sect. B Biol.Sci. (2012) 82:391-398DOI 10.1007/s40011-012-0042-0
Indigenous Techniques of Product Development and Economic Potential of Seabuckthorn: A Case Study of Cold Desert Region of Himachal Pradesh, India;
28. Gyan P Mishra Prospects of Local Flora of Trans-Himalayan Region of Ladakh for Various Medicinal Uses
29. Rashmi Gupta Efficacy of aqueous extract of Hippophae rhamnoides and its bio-active flavonoids against hypoxia-induced cell death Canadian Journal of Ophthalmology Journal Canadien D Ophtalmologie, Apr 1, 2006;
30. Vidya Rattan Anita Tomar Effect of Different Temperatures on the Seed Germination of Willow Leaf Seabuckthorn – Hippophae salicifolia, Forest Research Institute, Dehradun, Centre for Social Forestry and Eco-rehabilitation, Allahabad E-mail : anitatomar@rediffmail.com;
31. Anita Tomar, V.R.R. Singh and Vidya Rattan SEABUCKTHORN – A POTENTIAL BIORESOURCEIN HIMALAYAS FOR THE UPLIFTMENT OFLOCAL LIVELIHOOD Centre for Social Forestry and Eco-Rehabilitation, Allahabad - 211 001 Silviculture Division, Forest Research Institute, Dehradun - 248 006;
32. Rakshit Pathak ANTIOXIDANT PROFILING AND FATTY ACID COMPOSITION OF LIPIDS PRESENT IN HIPPOPHAE SALICIFOLIA GROWN IN HIGHER ALTITUDE OF UTTARAKHAND REGION;
33. Attar Singh Chauhan Antioxidant and antibacterial activities of various seabuckthorn ( L.) seed extracts;
34. Dipesh Pyakurel Effect of Temperature and Gibberellic Acid on Seed Germination and Seedling Growth of Seabuckthorn (Hippophae L. spp);  
35. Dr. Anita TomarSource variation in fruit, seed and seedling traits of Hippophae salicifolia;
36. Dipesh Pyakurel NTFPs: Impetus for Conservation and Livelihood support in Nepal. A Reference Book on Ecology, Conservation, Product Development and Economic Analysis of Selected NTFPs of Langtang Area in the Sacred Himalayan Landscape.
37. D. Mishra ANALYSIS AND CHARACTERIZATION OF RICE ENVIRONMENT OF ARUNACHAL PRADESH
38. Bhavana Sharma Ravi Gupta Renu Deswal MINING THE PROTEIN REPERTOIRE OF A HIMALAYAN SHRUB, HIPPOPHAE RHAMNOIDES FOR ANTIFREEZE PROTEINS;
39. Tadeusz Tylkowski Seed germination and seedling emergence in Hippophaë rhamnoides L, Dendrobiology 2010, vol. 63, 53–58;
40.  Anjana Bhatia Saroj Arora Bikram Singh Gurveen Kaur Avinash Nagpal Anticancer potential of Himalayan plants Phytochem Rev (2011) 10:309–323;     
41. Alessandro Boesi Traditional knowledge of wild food plants in a few Tibetan communities;
42. Bhakta Prasad Gaire and Lalita Subedi Medicinal Plant Diversity and their Pharmacological Aspects of Nepal Himalayas;
43. Rashmi Gupta Efficacy of aqueous extract of Hippophae rhamnoides and its bio-active flavonoids against hypoxia-induced cell death;
44. P.S. Negi, A.S. Chauhan, G.A. Sadi, Y.S. Rohinishree, R.S. Ramteke Antioxidant and antibacterial activities of various seabuckthorn ( L.) seed extracts    Human Resource Development Department, Central Food Technological Research Institute, Mysore 570 020, India, Fruit and Vegetable Technology Department, Central Food Technological Research Institute, Mysore 570 020, India www.elsevier.com/locate/foodchem;
46. Morvin Yabesh Quantitative ethnomedicinal study of plants used in the Skardu Valley at high altitude of Karakoram-Himalayan range, Pakistan, 2014, Journal of Ethnobiology and Ethnomedicine;
47. Satender Rana, Poonam Shirkot, M. C. Yadav A Female Sex Associated Randomly Amplified Polymorphic DNA Marker in Dioecious Hippophae salicifolia, Genes, Genomes and Genomics ©2009 Global Science Books;
48. Sadaf Kayani, Mushtaq Ahmad, Muhammad Zafar, Shazia Sultana ,Muhammad Pukhtoon Zada Khan, Muhammad Aqeel Ashraf, Javid Hussain GhulaYaseen Ethnobotanicalusesofmedicinalplantsforrespiratorydisorders among theinhabitantsofGallies – Abbottabad,NorthernPakistan;
49. Jan Oszmiański Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn ( Hippophaë rhamnoides L.) Berries Journal of Agricultural and Food Chemistry, 2015;
50. Somen Acharya Seabuckthorn (Hippophae sp. L.): New crop opportunity for biodiversity conservation in cold arid Trans-Himalayas;
51. Saurabh Chaudhary Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome;
52. Bhavana Sharma, Ravi Gupta, Renu Deswal Mining the protein repertoire of a Himalayan shrub, HIPPOPHAE RHAMNOIDES for antifreeze proteins Journal of proteins and proteomics 7(3), 2016, pp. 199-211;
53. Saurabh Chaudhary Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome 2013, Physiology and Molecular Biology of Plants;
54. Igor V Bartish An Ancient Medicinal Plant at the Crossroads of Modern Horticulture and Genetics: Genetic Resources and Biotechnology of Sea Buckthorn (Hippophae L., Elaeagnaceae hylogeography, Biotechnology,
Medicinal Plants, Genetic Diversity, DNA analysis
55. Dr. Anita Tomar V.R.R. Singh and Vidya Rattan SEABUCKTHORN – A POTENTIAL BIORESOURCE IN HIMALAYAS FOR THE UPLIFTMENT OF LOCAL LIVELIHOOD Centre for Social Forestry and Eco-Rehabilitation, Allahabad - 211 001 Silviculture Division, Forest Research Institute, Dehradun - 248 006
Stimate Domnule Dr.Ing.Angel Proorocu, 
Faptul ca ati primit din India o lucrare pe potriva experientei Dvs, inseamna recunoasterea internationala a valorii ca expert in problemele catinei. Felicitari sincere si ma bucur pentru succesul Dvs.
Cu deosebita consideratie,
Prof.Dr.Agatha Popescu
56. D. Dhyani, R.K. Maikhuri S. Misra, K.S. RaoEndorsing the declining indigenous ethnobotanical knowledge system of Seabuckthorn in Central Himalaya, India; journal homepage: www.elsevier.com/locate/jethpharm
"Fruit juice and oil of Seabuckthorn is a storehouse of nearly 190 bioactive substances and 106 components (Chu et al., 2003;Ranjith et al., 2006). Seabuckthorn plant is indigenous to the higher Himalayan region of Uttarakhand, but it is paradoxical that very few people are aware of its existence and tremendous multidimensional uses. Consequently, most of the resource perishes every year standing in the wild."
57. Shujaul Mulk Khan Saddam Khan Medicinal plants used by inhabitants of the Shigar Valley, Baltistan region of Karakorum range-Pakistan; Botany, Ethnobotany, Medicinal Plants, Karakorum Background: The inhabitants of mountainous terrains depend on folk therapies to treat various ailments; however lack of plant based research and geographical constraints set the traditional knowledge in jeopardy. Present study is the first documentation on traditional uses of plant species by the inhabitants of the Shigar Valley, Karakorum Range, Northern Pakistan. Method: Ethnobotanical data were collected over a period from July, 2013 to October, 2016 from 84 respondents, using semi structured questionnaire. Quantitative indices such as relative frequency citation (RFCs) and fidelity level (FL) were intended to evaluate the importance of medicinal plant species. Results: In total 84 plant species belonging to 36 families and 72 genera were recorded. Fabaceae was dominant with 7 species, followed by Asteraceae, Lamiaceae and Rosacea (6 species each). Leaves, root, flowers, seeds and fruits were the frequently utilized plant parts, whereas among drug formulations, decoction (49%) was ranked first. Majority of the plant species were used to treat abdominal, respiratory and dermal ailments (31, 12 and 12, respectively). RFCs value ranged 0.477 to 0.11 for Tanacetum falconeri and Allium carolinianum, respectively; while Hippophe rhamnoides and Thymus linearis depicted 100% FL. Comparative assessment with previous reports revealed that traditional uses of 26% plant species counting Hedyserum falconeri, Aconitum violoceum var. weileri, Arnebia guttata, Biebersteinia odora, Clematis alpine var. sibirica, Corydalis adiantifolia and Saussurea simpsoniana were reported for the first time. Conclusion: The endemic medicinal plant species and traditional knowledge of Balti community living in extremely high mountains area were explored for the first time. A comprehensive survey of this region could be significant to drive the existing knowledge in market circuit with sustainable collection, and to evaluate economic potential of the plant species. Additionally, social livelihood could be reinforced through establishing collection sites,transformation and drying centres for micro and macro marketing of medicinal plant species.
58. Arshad A Shedayi Traditional Medicinal use of Plants in Gilgit-Baltistan, Pakistan. Ethnobotany, Traditional Ecological Knowledge, Ethnobiology, Ethnomedicine, Ethnoecology. Journal of Medicinal Plant Research
59. Arshad Ali Shedayi and Bibi Gulshan Ethnomedicinal uses of plant resources in Gilgit-Baltistan of Pakistan Departement of Biological Sciences, Karakoram International University, Gilgit, Pakistan 2012. Hippophea rhamnoide L.(Asteraceae) Fruit, stem, and leaves. The fruits are used as cough syrups. After decoction of the berries are used for skin and lung problems. Also used for cancer, ulcer, wounds, skin infection, joint pain, hair fall, diabetes, blood pressure, jaundice and heart problems. Locally it is utilized as firewood and forage, particularly for goats.
60. Hilal YİLDİZ, Memnune SENGUL, Ferit CELİK, Sezai ERCİSLİ, Boris DURALİJA Bioactive Content of Sea Buckthorn (Hippophae rhamnoides L.) Berries from Turkey In Northeast part in urkey, wild grown fruits are abundant and they are widely col-lected and consumed by rural peoples. In this study, the fruits of seven sea buckthorngenotypes from Northeast in urkey were sampled and analyzed for their total phe-nolic content, vitamin C, total anthocyanins and antioxidant capacity. otal phenolic, vitamin C and total anthocyanin content varied from 213 to 262 mg GAE/100g FW;28-85 mg/100 g and 3-21 mg/L among genotypes. Antioxidant capacity analyses (inDPPH and β-carotene method) showed that all samples had a high antioxidant (aver-age 94.23% in β-Carotene and 31.23% in DPPH) capacity.
61. I. V. BARTISH, N. JEPPSSON and H. NYBOM Balsgarrd-Department of Horticultural Plant Breeding, Swedish University of Agricultural Sciences, Fjalkestadsvsgen 123D1, S-29194 Kristianstad, Sweden Population genetic structure in the dioecious pioneer plant species Hippophae rhamnoides investigated by random amplified polymorphic DNA (RAPD) markers Hippophae rhamnoides is an outcrossing pioneer plant species with a severely fragmented distribution. Random amplified polymorphic DNA (RAPD) marker variation was analysed in 10 populations of ssp. rhamnoides and in one population of ssp. mongolica to estimate the amount and distribution of genetic variability. No less than 89.7% of the scorable markers were polymorphic, but few of these were fixed and populations consequently differed mainly by frequency variation of individual markers. Within-population gene diversity was somewhat low for an outcrossing plant species: 0.192 or 0.159 for ssp. rhamnoides, depending on whether it was based on all 156 polymorphic RAPDs or on only those 63 RAPDs that fulfilled the 3/N criterion. Analysis of molecular variance applied to the ssp. rhamnoides showed only 15% between-population variability, indicating a relatively restricted population differentiation as expected in outcrossing species and shown in several other AMOVA studies. The tendency for island populations to be somewhat more differentiated, and to have less within-population diversity than mainland populations, may indicate an effect of population fragmentation. Genetic distance estimates among populations, obtained with and without pruning of RAPD loci on the basis of the 3/N criterion, were generally in very good agreement. Cluster analyses and principal coordinate analyses showed populations of ssp. rhamnoides to be rather close, but quite isolated from the single ssp. mongolica population. Genetic and geographical distances between the ssp. rhamnoides populations were not associated, indicating that large-scale geographical and ecotypic differentiation was not reflected in the RAPD profiles. 1999, Molecular Ecology.
62. Beejay Parajuli Aspects of traditional medicine in Nepal.
63. Saurabh Chaudhary Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome 2013, Physiology and Molecular Biology of Plants, Stress physiology and molecular biology of plants.
64. Zaheer Abbas , Shujaul Mulk Khan, Arshad Mehmood Abbasi, Andrea Pieroni, Zahid Ullah, Muhammad Iqbal and Zeeshan Ahmad Ethnobotany of the Balti community, Tormik valley, Karakorum range, Baltistan,Pakistan  Limited health facilities and malnutrition are major problems in the Karakorum Range of NorthernPakistan, often resulting in various human disorders. Since centuries, however, local communities in these areashave developed traditional methods for treating various ailments and local foods capes that can be significant fordevising public health and nutritional policies. This study was intended to document the ethnobotanicalknowledge of the local peoples in the Tormik Valley, especially in the medical and food domains. Field trips were undertaken in 14 different villages of the study area from 2010 to 2012. Ethnobotanical data were gathered using semi-structured interviews and group conversation with 69 informants. Details aboutlocal uses of plant species were recorded along with demographic characteristics of the visited communities.Relative frequency citation index (RFCi) and preference ranking index (PRi) tools were applied to determine the cultural significance of the reported species. Sixty-three plant species, with a predominance of Asteraceae and Fabaceae family members, as well astheir detailed folk uses were documented. Forty-three percent of the species were used to treat various diseases,21 % were consumed as wild fruits and vegetables and 53 % of the species had multipurpose applications. Thymuslinearis Benth, Hippophae rhamnoides ssp. Turkestanica L. and Convolvulus arvensis L. were found to be the most utilized medicinal plant species, i.e. those with significant RFCi values (0.54, 0.51 and 0.48, respectively). Betula utilis D. Don was the most versatile taxon (seven different ways of utilization); being this species a common and easily accessible subalpine tree and then under anthropogenic pressure, the implementation of concrete strategies aimedat its in-situ and ex-situ conservation is strongly recommended. The valleys in the Karakorum Mountains in the Northern Pakistan host significant Traditional Knowledge on local food and medicinal plant species, which need to be reconsidered and cautiously re-evaluated by ethnopharmacologists, and public health/nutrition actors. Furthermore, germane trans-disciplinary investigations are suggested to ensure the dynamic conservation of precious local knowledge systems, as well as plant diversity in Pakistani mountain regions.
65. Kuber P Bhatta, Yadav Uprety Traditional Practice and Knowledge of Indigenous and Local Communities in Kailash Sacred Landscape, Nepal Natural Resources, Cultural Landscapes, Traditional Ecological Knowledge, Indigenous Knowledge, Natural Resource Management. International Centre for Integrated Mountain Development (ICIMOD)All rights reserved, published 2017;


66. Raluca Maria Pop, Yannick Weesepoel, Carmen Socaciu, Adela Pintea, Jean-Paul Vincken, Harry Gruppen Carotenoid composition of berries and leaves from six Romanian seabuckthorn (Hippophae rhamnoides L.) varieties University of Agricultural Sciences and Veterinary Medicine, Ma˘ nastur Street, 3-5, 400372 Cluj-Napoca, Romania, Laboratory of Food Chemistry, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands journal homepage: www.elsevier.com/locate/foodchem;
67. Gyan P Mishra Prospects of Local Flora of Trans-Himalayan Region of Ladakh for Various Medicinal Uses - 12 Chapter 12:
Prospects of Local Floraof Trans-Himalayan Region of Ladakh forVarious Medicinal Uses
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DRDO, New Delhi.Singh, R., Mishra, G. P., Singh, N., and Singh, S. B. 2011.Protected cultivation and sustainable crop production athigh altitudes of Ladakh. Protected cultivation forsustainable horticulture. Ed. Sephia and Sharma,197–210.Dr YSPUHF, Nauni, HP, India.Stobdan, T., Chaurasia, O.P., Korekar, G., Mundra, S., Ali,Z., Yadav, A., and Singh, S. B. 2010. Attributes ofseabuckthorn (Hippophae rhamnoides L.) to meet nutritionalrequirements in high altitude. Defence Science Journal60(2): 226–230.Stobdan, T., Yadav, A., Mishra, G. P., Chaurasia, O. P.,and Srivastava, R. B. 2011. Seabuckthorn: The Super Plant(Production, Characterization, Postharvest and Healthapplications). 100. DIHAR, DRDO.Subhose, V., Srinivas P., and Narayana A. 2005. Basicprinciples of pharmaceutical science in Ayurveda. Bulletinof the Indian Institute of History of Medicine 35(2):83–92.Swaroop, A., Sinha, A. K., Chawla, R., Arora, R., Sharma,R. K., and Kumar, J. K. 2005. Isolation andCharacterization of 1,3-Dicapryloyl-2-linoleoylglycerol: ANovel Triglyceride from Berries of Hippophae rhamnoides.Chemical and Pharmaceutical Bulletin 53(8): 1021.Tantray, M. A., Tariq, K. A., Mir, M. M., Bhat, M. A., andShawl, A. S. 2009. Ethnomedicinal survey of Shopian,Kashmir (J&K), India. Asian Journal of TraditionalMedicines 4: 1–6.Tesoriere, L., Butera, D., Gentile, C., and Livrea, M. A.2007. Bioactive components of caper (Capparis spinosa L.)from Sicily and antioxidant effects in a red meat simulatedgastric digestion. Journal of Agricultural and FoodChemistry 55: 8465–8471.Tomas, F. and Ferreres, F. 1978. 3-O-Rhamnorutinosylkaempferol from floral buttons of Capparis spinosa.Revista de Agroquimica y Tecnologia de Alimentos 18:232–235.Trombetta, D., Occhiuto, F., Perri, D., Puglia, C.,Santagati, N. A., Pasquale, A. D., Saija, A., and Bonina,F. 2005. Anti-allergic and antihistaminic effect of twoextracts of Capparis spinosa L. flowering buds.
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68. Rajesh Ghangal, Saurabh Chaudhary, Mukesh Jain, Ram Singh Purty, Prakash Chand Sharma Optimization of De Novo Short Read Assembly of Seabuckthorn (Hippophae rhamnoides L.) Transcriptome
Abstract: Seabuckthorn (Hippophae rhamnoides L.) is known for its medicinal, nutritional and environmental importance sinceancient times. However, very limited efforts have been made to characterize the genome and transcriptome of thiswonder plant. Here, we report the use of next generation massive parallel sequencing technology (Illumina platform) and de novo assembly to gain a comprehensive view of the sea buckthorn transcriptome. We assembled 86,253,874high quality short reads using six assembly tools. At our hand, assembly of non-redundant short reads following a two-step procedure was found to be the best considering various assembly quality parameters. Initially, ABySS tool was used following an additive k-mer approach. The assembled transcripts were subsequently subjected to TGICLsuite. Finally, de novo short read assembly yielded 88,297 transcripts (> 100 bp), representing about 53 Mb of sea buckthorn transcriptome. The average length of transcripts was 610 bp, N50 length 1198 BP and 91% of the short reads uniquely mapped back to sea buckthorn transcriptome. A total of 41,340 (46.8%) transcripts showed significant similarity with sequences present in nr protein databases of NCBI (E-value < 1E-06). We also screened the assembled transcripts for the presence of transcription factors and simple sequence repeats. Our strategyinvolving the use of short read assembler (ABySS) followed by TGICL will be useful for the researchers working with a non-model organism’s transcriptome in terms of saving time and reducing complexity in data management. These a buckthorn transcriptome data generated here provide a valuable resource for gene discovery and development of functional molecular markers.
Citation: Ghangal R, Chaudhary S, Jain M, Purty RS, Chand Sharma P (2013) Optimization of De Novo Short Read Assembly of Seabuckthorn( Hippophae rhamnoides L.) Transcriptome. PLoS ONE 8(8): e72516. doi: 10.1371/journal.pone.0072516
Editor: Qiong Wu, Harbin Institute of Technology, China Published August 21, 2013
Copyright: © 2013 Ghangal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was financially supported by Department of Biotechnology, Government of India, New Delhi (BT/PR10798/NDB/51/170/2008); http://dbtindia.nic.in/index.asp. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests:  The authors have declared that no competing interests exist.* E-mail: prof.pcsharma@gmail.com
69.  Shujaul Mulk Khan, Zeeshan Ahmad Ethnobotany of the Balti community, Tormik valley, Karakorum range, Baltistan, Pakistan
Limited health facilities and malnutrition are major problems in the Karakorum Range of NorthernPakistan, often resulting in various human disorders. Since centuries, however, local communities in these areashave developed traditional methods for treating various ailments and local foods capes that can be significant fordevising public health and nutritional policies. This study was intended to document the ethnobotanicalknowledge of the local peoples in the Tormik Valley, especially in the medical and food domains. Field trips were undertaken in 14 different villages of the study area from 2010 to 2012. Ethnobotanicaldata were gathered using semi-structured interviews and group conversation with 69 informants. Details aboutlocal uses of plant species were recorded along with demographic characteristics of the visited communities.Relative frequency citation index (RFCi) and preference ranking index (PRi) tools were applied to determine thecultural significance of the reported species. Sixty-three plant species, with a predominance of Asteraceae and Fabaceae family members, as well astheir detailed folk uses were documented. Forty-three percent of the species were used to treat various diseases,21 % were consumed as wild fruits and vegetables and 53 % of the species had multipurpose applications. Thymuslinearis, Benth, Hippophae rhamnoides ssp. Turkestanica L. and Convolvulus arvensis L. were found to be the mostutilized medicinal plant species, i.e. those with significant RFCi values (0.54, 0.51 and 0.48, respectively). Betula utilis D. Don was the most versatile taxon (seven different ways of utilization); being this species a common and easilyaccessible subalpine tree and then under anthropogenic pressure, the implementation of concrete strategies aimedat its in-situ and ex-situ conservation is strongly recommended. Conclusion: The valleys in the Karakorum Mountains in the Northern Pakistan host significant Traditional Knowledge on local food and medicinal plant species, which need to be reconsidered and cautiously re-evaluated by ethnopharmacologists, and public health/nutrition actors. Furthermore, germane trans-disciplinary investigationsare suggested to ensure the dynamic conservation of precious local knowledge systems, as well as plant diversity inPakistani mountain regions. Keywords:  Ethnobotany, Medicinal plants, Indigenous knowledge, Karakorum, Pakistan Correspondence: shuja60@gmail.com; arshad799@yahoo.com 2 Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan 3 Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, PakistanFull list of author information is available at the end of the article
© 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link tothe Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/ ) applies to the data made available in this article, unless otherwise stated. Abbas et al. Journal of Ethnobiology and Ethnomedicine (2016) 12:38 DOI 10.1186/s13002-016-0114-y\
70. Bhakta Prasad Gaire and Lalita Subedi Department of Herbology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea. (samarpanbp@gmail.com) Department of Pharmaceutical Science, School of Health and Allied Sciences, Pokhara University,PO Box. 427, Kaski, Nepal (subedilali@gmail.com) Medicinal Plant Diversity and their Pharmacological Aspects of Nepal Himalayas Background: The Himalayan range of Nepal is affluent with vast diversity of medicinal plants. Due to insufficient supplement of modern allopathic medicine and the traditional believe of ethnomedicinal therapy, still vast majority of Nepalese people are dependent on indigenous use of medicinal plant. Use of Nepalese Himalayan medicinal plants is not only limited to erogenous use of Nepal Himalaya but also regarded as chief ingredients in Eastern medicinal system including Ayurveda of Indian subcontinent, Traditional Chinese Medicine, Korean Oriental Medicine, etc. But due to the lack of efficient pharmacological investigation, Himalayan plant diversity is still limited to their ethnomedicinal uses. Vigorous pharmacological investigation is mandatory to explore their therapeutic potential. Conclusion: Here in this review; based on latest published pharmacological research articles, we tried to explore pharmacological aspects of major Himalayan medicinal plant of Nepal for the first time. There is the current need to investigate further pharmacological potency of these medicinal plants in order to explore their therapeutic potential.
71. Tadeusz Tylkowski Seed germination and seedling emergence in Hippophaë rhamnoides L In contrast to earlier reports, in this study Seabuckthorn seeds collected from ripe fruits proved tobe non-dormant. Most of them germinated quickly at high temperatures (e.g. cyclically alternating tempera-turesof 20~30°C daily for 16 and 8 hours,respectively).Atlowertemperatures(3~20°C),seedsdidnotger-minate or germinated slowly, but stratification at 3°C for 4–6 weeks increased their germination rate to90–100% within 2–3 weeks. Seedling emergence  in the open ursery proved to berisky,evenafter6–8  weeks of seed stratification, so spring sowing in the greenhouse (or plastic tunnel) is recommended. Additional keywords: seed dormancy; stratification; propagation from seeds; Seabuckthorn Address: Polish Academy of Sciences; Institute of Dendrology; Parkowa 5,62-035 Kórnik, Poland, e-mail:ttylkows@man.poznan.pl References: Bärtels A. 1982. Rozmnażanie drzew i krzewówozdobnych. PWRiL, Warszawa.Bewley J.D. 1997. Seed germination and dormancy. The Plant Cell 9: 1055–1066.Bieniek A., Kawecki Z., Szałkiewicz M. 2007. Plono-wanie kilku odmian rokitnika zwyczajnego (Hippophae rhamnoides L.) w warunkach Warmii.Roczniki Akademii Rolniczej w Poznaniu 383:275–278.Bonner F.T. 1984. Glossary of seed germinationterms for tree seed workers. Gen. Tech. Rep.SO-49. New Orleans: USDA Forest Service,Southern Forest Experiment Station. 4 p.Cram W.H., Nagv M.J., Lindquist C.H. 1960. Propa-gation research. In: 1960 Summary report for theForest Nursery Station. Indian Head, SK: CanadaDepartment of Agriculture, Research Branch:16–18.Dhyani D., Maikhuri R.K., Rao K.S., Kumar L., Pu-rohit V.K., Sundriyal M., Saxena K.G. 2007. Basicnutritional attributes of  Hippophae rhamnoides (Seabuckthorn) populations from UttarakhandHimalaya,India.CurrentScience92:11481152.DirrM.A.,HeuserC.W.Jr.2006.TheReferenceMan-ual of Woody Plant Propagation: From Seed to Tissue Culture. Varsity Press, Inc., Cary, NorthCarolina.Giejbowicz E., Wołek T. 2008. Rokitnik zwyczajny jako przykład uprawy innowacyjnej: perspektywyrozwoju produkcji, przetwórstwa i rynku konsu-mentawPolsce.SGGWKatedraPolitykiAgrarneji Marketingu, Innowacje i innowacyjność w sek-torze Agrobiznesu Prace Naukowe nr 45: 69–80.Gosling P. 2007. Raising trees and shrubs from seed.ForestryCommissionPracticeGuide18.ForestryCommission, Edinburgh.Hawker L.S., Fraymouth J. 1951. A Re-investigationof the root-nodules of species of Elaeagnus, Hip- pophae, Alnus and Myrica, with special reference tothe morphology and life histories of the causativeorganisms. Journal of General Microbiology 5:369–386.Kanayama Y., Ohkawa W., Chiba E., Sato K., Kana-hama K., Ofosu-Anim J. 2009. Nutritional com-ponents and nitrogen fixation in seabuckthorn (Hippophae rhamnoides L.). Acta Horticulturae 806:309–314. http://www.actahort.org/books/806/ 806_38.htm.Leadem C.L. 1997. Dormancy-Unlocking Seed Se-crets. In: Landis T.D., Thompson J.R., tech.coords.NationalProceedings,ForestandConser-vation Nursery Associations. Gen. Tech. Rep.PNWG TR-419. Portland, OR: U.S. Departmentof Agriculture, Forest Service, Pacific NorthwestResearch Station. 43–52.Macdonald B. 1986. Practical woody plant propaga-tion for nursery growers. Timber Press (OR).Noculak-Palczewska A., Rykowski P. 2003. Rokitnikzwyczajny Hippophae rhamnoides L. i jego zastoso- wanie w kosmetyce. Polish Journal of Cosmeto-logy 6: 35–43.Pearson M.C., Rogers J.A. 1962. Hippophae rhamnoides L. Journal of Ecology 50: 501–513.Piotto B., Di Noi A. 2003. Seed propagation of Medi-terranean trees and shrubs. Manuali e linee gui-da. APAT. 16: 31.Qin H., Gilbert M.G. 2007. Eleagnaceae. In: Wu Z.Y.,Raven P.H., Hong D.Y. (eds.) Flora of China. Vol.13: 251–273. Science Press, Beijing and MissouriBotanical Garden Press, St. Louis.SabirS.M.,MaqsoodH.,HayatM.,KhanM.Q.,Khaliq A.2005. 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Seed storage, germination andseedling emergence in Rhamnus catharticus. Den-drobiology 58: 67–72.WernerT.2002.Gospodarstwopełnenowości.HasłoOgrodnicze 12.Zeb A. 2006. Anticarcinogenic Potential of Lipidsfrom Hippophae – Evidence from the Recent Liter-ature. Asian Pacific Journal of Cancer Prevention7: 32–35.

72. Arshad Ali Shedayi, Ming Xu, Bibi Gulraiz Traditional medicinal uses of plants in Gilgit-Baltistan, Pakistan As also mentioned by Luitel et al. (2014), the highest number of plant species was used for gastrointestinal-related diseases followed by cuts and wounds,and fever. Artemisia maritima, Artemisia annum and Hippophea rhamnoide are used to treat more than one disease (Shedayi and Gulshan, 2012);

73. A. Ranjith, K. Sarin Kumar, V.V. Venugopalan, C. Arumughan, R.C. Sawhney, and Virendra Singh Fatty acids, tocols, and carotenoids in pulp oil of three sea buckthorn species (Hippophae rhamnoides, H. salicifolia, and H. tibetana) grown in the Indian Himalayas Seabuckthorn berries from Hippophae rham- noides, H. tibetana, and H. salicifolia were collected from thecold deserts of the Himalayas (Lahaul, Ladakh, and Spiti; India)and characterized in terms of the FA, carotenoid, tocopherol, andtocotrienol composition in their pulp oil. These varied fromspecies to species. Total carotenoids ranged from 692 to 3420mg/kg in pulp oils of fresh berries, and total tocols, from 666 to1788 mg/kg. Hippophae salicifolia berries contained substantiallylower amounts of pulp oil, with lower levels of carotenoids andtocopherols. There was little difference in the proportion of indi-vidual tocols in pulp among the three species. α -Tocopherolalone constituted 40–60% of total pulp tocols in berries. Pulp oilshad palmitoleic acid (32–53%) as the most abundant FA followedby palmitic (25–35%), oleic (8–26%), linoleic (5–16%), andlinolenic (0.6–2.6%) acids, with the highest deviation observedin the proportion of palmitoleic acid in these berries.  Hippophae rhamnoides and H. tibetana contained the highest amount of thelipophilic carotenoids and tocols. Hippophae salicifolia berrieshad higher amounts of lipophobic constituents such as vitamin C and flavonols.

74. Sadaf Kayani, Mushtaq Ahmad, Muhammad Zafar, Shazia Sultana, Muhammad Pukhtoon Zada Khan, Muhammad Aqeel Ashraf, Javid Hussain, Ghulam Yaseen Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies

75. Vidya Rattan and Anita Tomar (Forest Research Institute, Dehradun Centre for Social Forestry and Eco-rehabilitation, Allahabad E-mail: anitatomar@rediffmail.com) Effect of Different Temperatures on the Seed Germination of Willow Leaf Seabuckthorn – Hippophae salicifolia

 Hippophae salicifolia D. Don (Vernacular - Chuk.Tarwa) is a deciduous tree species restricted to theHimalayan region, between 1500-3500 m a. m. s. l. (Hooker, 1894 and Gaur 1999). It has been reported as one of the best species of genus Hippophae interms of high-quality fruit, high yield and less thorns (Lu et al. 2001). There are total five species based on morphological variations viz. H. rhamnoides L., H.salicifolia D. Don, H. neurocarpa Liu & He, H. tibetana Schlecht and H. goniocarpa. The main species of Seabuckthorn distributed in India are H. rhamnoides, H. salicifolia and H. tibetana. Out of these species, only two species, i.e., H. salicifolia D. Don and H. tibetana S. are reported in Uttarakhand (Yadav et al. , 2006 a).Among them H. salicifolia is the most common and widely distributed species and also called as willowleaf or arboreal is reported to exist in abundance inthree districts of Uttarakhand viz., Uttarkashi,Chamoli and Pithoragarh (Yadav et al. ,2006 b).The uses of Seabuckthorn are manifold  Hippophae salicifolia D. Don (Vernacular - Chuk.Tarwa) is a deciduous tree species restricted to theHimalayan region, between 1500-3500 m a. m. s. l.(Hooker, 1894 and Gaur 1999). It has been reported as one of the best species of genus Hippophae interms of high quality fruit, high yield and less thorns (Lu et al., 2001). There are total five species based onmorphological variations viz. H. rhamnoides L., H.salicifolia D. Don, H. neurocarpa Liu & He, H. tibetana Schlecht and H. goniocarpa. The main species of Seabuckthorn distributed in India are H. rhamnoides, H. salicifolia and H. tibetana. Out of these species, onlytwo species, i.e., H. salicifolia D. Don and H. tibetana S.are reported in Uttarakhand (Yadav et al., 2006 a).Among them H. salicifolia is the most common and widely distributed species and also called as willow leaf or arboreal is reported to exist in abundance inthree districts of Uttarakhand viz., Uttarkashi,Chamoli and Pithoragarh (Yadav et al. ,2006 b).The uses of Seabuckthorn are manifold and include its use as fence, fuel, fodder, fruit and medicines. It is used for protection against wild animal and is used as bio-fence around houses and cultivated fields. This species has great ecologicalsignificance as its roots possess excellent soilbinding properties. Frankia present in its rootnodules fixes atmospheric nitrogen @180kg/ha/annum. Natural forest of Seabuckthorn can yield 750-1,500 kg of berries/ha and berries are the richsource of vitamin C, caretonoids, minerals, vitaminB, vitamin E and vitamin K. Seeds contain highquality oil which has many bioactive substances(Lu,1992).The fruits have a distinctive sours taste and aunique aroma reminiscent of pineapple. The localpeoples of Uttarakhand state who leaved nearby, thehabitat of H. salicifolia, they used berries for pickles.The berries remain on the small tree branches allwinter until eaten by birds. Scientifically the qualityof fruit was recorded as a rich source of vitamins, and used in preparations of various products includinglocal beverages (Gaur,1999).

76. Ankit Jain, Saurabh Chaudhary, Prakash Chand Sharma Mining of microsatellites using next generation sequencing of seabuckthorn (Hippophae rhamnoides L.) transcriptome.

77. Handesh Bhattarai, Ram P Chaudhary, Cassandra L Quave, Robin SL Taylor The use of medicinal plants in the trans-himalayan arid zone of Mustang district, Nepal Bhattarai Journal of Ethnobiology and Ethnomedicine 2010,6:14http://www.ethnobiomed.com/content/6/1/14 The vegetation of Mustang has been categorized into8 types namely: mixed forest (Pinus wallichiana forest, Betula utilis forest, Hippophae salicifolia forest, Cara- gana gerardiana forest, Caragana gerardiana and Lonicera spinosa forest, Juniperus forest) and grasslands withpure stocks of Poaceae [21]. The area is characterizedby the high altitude, cold climate, semi-desert environ-ment [22], with altitudinal variations of 1,500 to 8,000m.a.s.l. The district has characteristic vegetation with afreezing season of about 73-119 days (Marpha-Lo-Man-thang) [23], and is dominated by shrubby and dwarf plant communities [24]. The influence of such charac-teristic environmental conditions in the Himalayanregion including Mustang established favourable growthconditions for some of the medicinal plant species at altitudes as high as 6000-6300 m.a.s.l.

78. Jan Oszmiański Analysis of Lipophilic and Hydrophilic Bioactive Compounds Content in Sea Buckthorn ( Hippophaë rhamnoides L.) Berries

Mirosława Teleszko, Aneta Wojdyło, Magdalena Rudzinska, Jan Oszmianski and Tomasz Golis

Department of Fruit and Vegetable Technology, Wrocław University of Environmental and Life Sciences, Chełmonskiego 37 Street, 5 51 630 Wrocław, Poland, 6, Institute of Food Technology of Plant Origin, PoznanUniversity of Life Sciences, Wojska Polskiego 31 Street, 60 624 Poznan,7 Poland 8 Department of Pomology, Gene Resources and Nurseries, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Street, 96-1009 Skierniewice, Poland,10

 ABSTRACT:

 The aim of this study was to determine selected phytochemicals in berries of eight sea buckthorn (Hippophae rhamnoides subsp. mongolica) cultivars, including lipophilic and hydrophilic compounds. In the experiment chromatographic analyses, GC (phytosterols and fatty acids), UPLC-PDA-FL, LC-MS (polyphenols), and HPLC (L-ascorbic acid), as wellspectrophotometric method (total carotenoids) were used. The lipid fraction isolated from whole fruit contained 14 phytosterols (major compounds β-sitosterol > 24-methylenecykloartanol > squalene) and 11 fatty acids in the order MUFAs > SFAs > PUFAs. Carotenoids occurred in concentrations between 6.19 and 23.91 mg/100 g fresh weight (fw) (p < 0.05). The major polyphenol group identied in berries was avonols (mean content of 311.55 mg/100 g fw), with the structures of isorhamnetin (six compounds), quercetin (four compounds), and kaempferol (one compound) glycosides. Examined sea buckthorn cultivars were characterized also by a high content of L-ascorbic acid in a range from 52.86 to 130.97 mg/100 g fw (p< 0.05). KEYWORDS: sea buckthorn, bioactive compounds, fatty acids, phytosterols, polyphenols.




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